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Glutathione Agarose Resin 5 ml

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Glutathione Agarose Resin

Glutathione agarose resin has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

Glutathione-agarose provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Bound GST fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione.

Do not autoclave

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  Bead (Geometry, size)   Spherical, Standard: ~ 50-150 μm
  Cross linking   No
  Ligand   Glutathione, linked via sulphur atom
  Spacer Arm   12 atoms
  Binding Capacity   > 8 mg recombinant GST / ml gel
  Applications   Batch, gravity, MPLC and FPLC
  FTIR   Reference spectrum


1 ml gel corresponds to 1.333 ml of original 75% suspension)

Glutathione Agarose Recommend Flow Rates

  Bed Volume
  Inner diameter
  Sample loading
  1 ml
  6.6 mm
  0.2 – 1.0 ml/min
  1.0 ml/min
  10 ml
  16 mm
  0.5 – 5.0 ml/min
  5.0 ml/min

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