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Glutathione Agarose Resin
Glutathione agarose resin has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.
Glutathione-agarose provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Bound GST fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione.
Do not autoclave
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| Bead (Geometry, size) | Spherical, Standard: ~ 50-150 μm |
| Cross linking | No |
| Ligand | Glutathione, linked via sulphur atom |
| Spacer Arm | 12 atoms |
| Binding Capacity | > 8 mg recombinant GST / ml gel |
| Applications | Batch, gravity, MPLC and FPLC |
| FTIR | Reference spectrum |
1 ml gel corresponds to 1.333 ml of original 75% suspension)
Glutathione Agarose Recommend Flow Rates
| Bed Volume |
Inner diameter
|
Sample loading
|
Washing/Elution
|
| 1 ml |
6.6 mm
|
0.2 – 1.0 ml/min
|
1.0 ml/min
|
| 10 ml |
16 mm
|
0.5 – 5.0 ml/min
|
5.0 ml/min
|
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