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Protein A Resin 5 ml


Protein A Resin

(Protein A agarose resin)

Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

We offer 3 levels of binding capacity:

  • Basic ~20 mg Porcine IgG/ml
  • Standard ~25 mg hIgG/m
  • Ultra: 30-40 mg hIgG/ml

The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

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