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Anti-α-Adaptin Monoclonal
| Clone | Ada-1 |
| Size | 100μg/vial |
| Form | lyophilized |
| Ig type | mouse IgG2a |
| Immunogen | AP-2 adaptor polypeptides from bovine brain. |
| Application | WB |
| Cross Reactivity | Human, rat. |
| Reconstitution | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, |
| the antibody concentration will be 100μg/ml. | |
| Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
| It can also be aliquotted and stored frozen at -20˚C for a longer time. | |
| Avoid repeated freezing and thawing. | |
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Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. . Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels. |
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