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ABTS diammonium salt 1 g


ABTS diammonium salt

(abts diammonium salt; Ethylmercurithiosalicylic acid sodium salt)

Purity > 98.0%

  CAS Number:
  Chemical Formula:
  Molecular Weight:   548.68 g/mol


ABST diammonium salt is commonly used as a substrate in biochemical assays, enzymatic kinetics and ELISA experiments.  ABST becomes green in color when converted to its monocationic radical (ABST+).  This change can be monitored by spectrophotometric measurements at 405 nm. 

Hydrogen peroxide often serves as the oxidizing agent for this color change.  Indirectly monitoring hydrogen peroxide concentrations is beneficial due to its implications in the metabolism of free radicals.

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Celebrity Endorsements

Gallati, V. H. (1979) Horseradish peroxidase: a study of the kinetics and the determination of optimal reaction conditions, using hydrogen peroxide and 2,2'-azinobis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) as substrates, J. Clin. Chem. Clin. Biochem. 17 (1), S. 1-7.

Porstmann, B., et al., (1981) Comparison of chromogens for the determination of horseradish peroxidase as a marker in enzyme immunoassay, J. Clin. Chem. Clin. Biochem. 19 (7), S. 435-439.

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