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2xYT Mix 500 g


2xYT Mix

(2xYT Media)

2xYT medium is a standard nutrient media for maintaining and propagating Escherichia coli and M13 bacteriophage.

Formula: per liter: Peptone 20g, Yeast Extract 10g, Glucose 20g.
Usage: dissolve 50g of mix in 1 liter of water, adjust PH to 6.8, autoclave at 121oC for 15 min.
Storage: in tightly-closed container at room teperature.


  • NaCl 5 g
  • Tryptone 16 g
  • Yeast Extract 10 g


Dissolve 31 g of mix in 1 liter of water, adjust pH if desired, autoclave to sterilize.


  • Tightly closed at room temperature.


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