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X-Gal
(5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside)
Purity > 99.0 %
Molecular Biology Grade
| CAS Number: |
7240-90-6
|
| Chemical Formula: |
C14H15BrClNO6
|
| Molecular Weight: |
408.63 g/mol
|
The substrate X-gal is metabolized by the enzyme β-galactosidase into an insoluble blue precipitate.
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Protocols Using X-Gal:
How to make a 20 mg/ml X-Gal Stock Solution
1. Weigh 200 mg of X-Gal into a 15 ml polypropylene centrifuge tube.
2. Add 10 mL of Dimethylformamide (DMF). Vortex to dissolve the X-Gal.
3. Store at -20°C. Wrap the centrifuge tube in aluminum foil to protect from light.
Note: The X-Gal solution is stable for 6-12 months unless improperly handled. We recommend making 1 ml aliquot portions of the solution to minimize degradation if there will be frequent small scale use of the X-Gal solution.
Blue/White Screening of Bacterial Colonies X-Gal/IPTG Plates
View this method on protocols.io and use it directly at your bench
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| Acros Organics | 22777-1000 |
| Acros Organics | 32724-1000 |
| Alfa Aesar | B21034-MD |
| Alfa Aesar | B21034-MA |
| Alfa Aesar | 2264 |
| Alfa Aesar | B21034-MF |
| Amresco | E708-1ML |
| Amresco | 0487-1G |
| Amresco | N714-10ML |
| Amresco | 0428-1G |
| Amresco | 0487-10G |
| Amresco | N714-1.5ML |
| Amresco | 0428-100MG |
| Amresco | 0487-100G |
| Avantor | V261-01 |
| Bachem | M-1190.0250 |
| Bachem | M-1190.1000 |
| Bachem Americas | M-1190.5000 |
| EMD Millipore | 9660-1GM |
| Grow Cells | MESP-5003 |
| IBI Scientific | IB02265 |
| MP Biomedicals | 15000190 |
| Matrix Scientific | 008823-250MG |
| Matrix Scientific | 008823-1G |
| TCI America | B3201-1G |
| TCI America | B3201-200MG |
| Toronto Research Chemicals | B6823501G |
| Whatman | 2260 |
Celebrity Endorsements
Day, Kenneth , Gabi Shefer, Andrew Shearer, and Zipora Yablonka-Reuveni. "The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny." Science Direct 340.2 (2010): 330-343.
Hashizume, Rintaro, Nalin Gupta, Mitchel S Berger, Ann Banerjee, Michael D Prados, Jennifer Ayers-Ringler, C David James, and Scott R VandenBerg. "Morphologic and molecular characterization of ATRT xenografts adapted for orthotopic therapeutic testing." Neuro-Oncology 12.4 (2010): 366-376.
Low, H., Greco, B., Tanahashi, Y., Gallant, J., Jones, S., Billings-Gagliardi, S., Recht, L. and Schwatz, W. "Embryonic Stem Cell Rescue of Tremor and Ataxia in Myelin-deficient Shiverer Mice." Journal of the Neurological Sciences 276.1 (2009): 133.
Maricich, Stephen M, Scott A Wellnitz, Aislyn M Nelson, Daine R Lesniak, Grepory J Gerling, Ellen A Lumpkin, and Huda Zoghbi. "Merkel Cells Are Essential for Light-Touch Responses." Science 324 (2009): 1580.
H. Low, B. Greco, Y. Tanahashi, J. Gallant, S. Jones, S. Billings-Gagliardi, L. Recht, W. Schwartz. "Embryonic stem cell rescue of tremor and ataxia in myelin-deficient shiverer mice." Journal of the Neurological Sciences 276.1 (2009): 133-137
Sameer Phalke, Olaf Nickel, Diana Walluscheck, Frank Hortig, Maria Cristina Onorati, Gunter Reuter. "Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2." Nature Genetics 46 (2009): 696-702.
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