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X Gal 1 g




Purity > 99.0 %

Molecular Biology Grade

  CAS Number:
  Chemical Formula:
  Molecular Weight:
  408.63 g/mol


The substrate X-gal is metabolized by the enzyme β-galactosidase into an insoluble blue precipitate.

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Protocols Using X-Gal:

How to make a 20 mg/ml X-Gal Stock Solution

1. Weigh 200 mg of X-Gal into a 15 ml polypropylene centrifuge tube.
2. Add 10 mL of Dimethylformamide (DMF). Vortex to dissolve the X-Gal. 
3. Store at -20°C. Wrap the centrifuge tube in aluminum foil to protect from light.

Note: The X-Gal solution is stable for 6-12 months unless improperly handled. We recommend making 1 ml aliquot portions of the solution to minimize degradation if there will be frequent small scale use of the X-Gal solution.

Blue/White Screening of Bacterial Colonies X-Gal/IPTG Plates

View this method on protocols.io and use it directly at your bench

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Celebrity Endorsements

Cromie, Michael J, and Eduardo A. Groisman. "Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica." Journal of Bacteriology 192.2 (2010): 604-607.

Day, Kenneth , Gabi Shefer, Andrew Shearer, and Zipora Yablonka-Reuveni. "The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny." Science Direct 340.2 (2010): 330-343.

Hashizume, Rintaro, Nalin Gupta, Mitchel S Berger, Ann Banerjee, Michael D Prados, Jennifer Ayers-Ringler, C David James, and Scott R VandenBerg. "Morphologic and molecular characterization of ATRT xenografts adapted for orthotopic therapeutic testing." Neuro-Oncology 12.4 (2010): 366-376.

Low, H., Greco, B., Tanahashi, Y., Gallant, J., Jones, S., Billings-Gagliardi, S., Recht, L. and Schwatz, W. "Embryonic Stem Cell Rescue of Tremor and Ataxia in Myelin-deficient Shiverer Mice." Journal of the Neurological Sciences 276.1 (2009): 133.

Maricich, Stephen M, Scott A Wellnitz, Aislyn M Nelson, Daine R Lesniak, Grepory J Gerling, Ellen A Lumpkin, and Huda Zoghbi. "Merkel Cells Are Essential for Light-Touch Responses." Science 324 (2009): 1580.

H. Low, B. Greco, Y. Tanahashi, J. Gallant, S. Jones, S. Billings-Gagliardi, L. Recht, W. Schwartz. "Embryonic stem cell rescue of tremor and ataxia in myelin-deficient shiverer mice." Journal of the Neurological Sciences 276.1 (2009): 133-137

Sameer Phalke, Olaf Nickel, Diana Walluscheck, Frank Hortig, Maria Cristina Onorati, Gunter Reuter. "Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2." Nature Genetics 46 (2009): 696-702.

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