Maxiprep Kit

The UltraClean® Maxi Plasmid Prep Kit is designed to isolate plasmid DNA from large culture volumes of E. coli. The Maxi Kit uses the same silica spin filter technology employed in the UltraClean® 6 Minute Mini Plasmid Prep Kit. Unlike other kits, this maxi plasmid prep kit can be used with LB Broth culture as well as high nutrient broth culture media including Terrific Broth, TB DRY®, and 2X YT. The recommended starting volume is 250 ml of culture.

The procedure is to lyse cells with an improved set of alkaline lysis reagents. The DNA is bound to a silica spin filter, washed once and released into a low volume of elution buffer. The recovered DNA is concentrated enough to be used directly in automated sequencing. The DNA is also ready for restriction digests, labeling, PCR and other downstream applications.

• Rapid protocol isolates high quality plasmid DNA in 45 minutes

• Yields high quality DNA, ready for restriction digest, sequencing, labeling or PCR

• Easy format utilizes large silica spin filters

• High yields from standard or high nutrient broth culture

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