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DTNB 25 g

Ships in 5-10 days


(5,5'-Dithio-bis(2-nitrobenzoic acid); Ellman’s Reagent; 3-Carboxy-4-nitrophenyl disulfide; 6,6′-Dinitro-3,3′-dithiodibenzoic acid; Bis(3-carboxy-4-nitrophenyl) disulfide)

Purity > 99.0 %

  CAS Number:
  Chemical Formula:   C14H8N2O8S2
  Molecular Weight:
  396.35 g/mol


DTNB is a sensitive reagent that is commonly used for measuring the free sulfhydryl content in proteins, peptides, and tissues.

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Celebrity Endorsements

Santhiago, M; et. al. (2010) An amperometric sensor for l-cysteine based on nanostructured platform modified with 5, 5'-dithiobis-2-nitrobenzoic acid (DTNB). Sensors and Actuators B: Chemical. Vol 146, 213-220.

-In this paper, the activation of 5,5-dithiobis-2-nitrobenzoic acid (DTNB) in situ is proposed, for l-cysteine electrooxidation at low overpotentials.

Riddles, P.W.; et. al. (1979) Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination. Analytical Biochemistry. Vol 94. 75-81.

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