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MUG 1 g

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(Mug; 4-Methylumbelliferyl-B-D-glucuronide; 4-Methylumbelliferyl beta-D-glucuronide)

Purity > 99.0 %

  CAS Number:
  Chemical Formula:   C16H16O9
  Molecular Weight:
  352.3 g/mol


MUG is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase (GUR), which is encoded by uidA. GUR activity is tested in minimal medium o-nitrophenyl-beta-D-galactopyranoside (MMO)-MUG.  Fluorescence assay allows quantitation of GUS activity in protein extracts at peak excitation of 365 nm (UV) and a peak emission of 455 nm (blue).

Celebrity Endorsements

Jefferson, R.A. (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reporter. Volume 5, Number 4, 387-405.

-Discussion relating to the fluorogenic monitoring of gene expression including assay information utilizing MUG.

Sanderson, S.; Shastri, N. (1994) LacZ inducible, antigen/MHC-specific T cell hybrids. International Immunology, Vol 6, No 3, 369-376.

-The lacZ activity was obtained with the fluorescent MUG substrate which was 3 to 10-fold better than CPRG (chlorophenol red beta-galactoside) substrate during their experimental assays.

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