CSF2, Human

(Recombinant Human Colony Stimulating Factor 2 (granulocyte-macrophage) CSF2, GM-CSF, GMCSF Molgramostin, MGI-1GM (Macrophage granulocyte inducer-1GM))

Purity: >96% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

Colony Stimulating Factor 2 (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors.

It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. CSF2 is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

On mature hematopoietic cells, CSF2 is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. It can induce human endothelial cells to migrate and proliferate and can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. CSF2 is species specific and human CSF2 has no biological effects on mouse cells.

rHuCSF2 General Information
Amino Acid Sequence (128 aa):
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions..

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

Free Shipping within the Continental USA