CSF3, Human

(Recombinant Human Colony Stimulating Factor 3, CSF3, G-CSF, Granulocyte Colony-Stimulating Factor, Pluripoietin)

Purity: >98% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

Colony Stimulating Factor 3 (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage.

It is mainly produced by monocytes and macrophages upon activation by endotoxins. Various carcinoma cell lines and myeloblastic leukemia cells can express CSF3 constitutively. In humans, two distinct cDNA clones for CSF3 have been isolated. Both proteins have a 30 amino acid signal peptide and have nearly identical amino acid sequences.

Human CSF3 is 73% identical at the amino acid level to murine CSF3 and the two proteins show species cross-reactivity.

rHuCSF3 General Information
Amino Acid Sequence (174 aa):
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL PTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions.

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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