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Tween 20 500 ml


Tween 20

(Polysorbate 20; PEG(20)sorbitan monolaurate; Polyoxyethylene (20) sorbitan monolaurate; Alkest TW 20)

  CAS Number:
  Chemical Formula:
  Molecular Weight:   1227.54 g/mol


Tween 20 is commonly used as a detergent with applications as a washing agent in immunoassays such as in western blotting and ELISAs.  The reason is to help non-specific antibody interactions and used at concentrations of 0.05% to 0.5% v/v.  Tween 20 is also used for cell lysis, protein extraction and to solubilize membrane proteins.

Tween 20 at elevated temperatures is not recommended including autoclaving.

DNase, Protease and RNase Free

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Celebrity Endorsements

Pautsch, A., et. al. (1999) Strategy for membrane protein crystallization exemplified with OmpA and OmpX. Proteins: Structure, Function, and Bioinformatics. Vol 34. 167–172.

Lin, C.Y., et al. (2010) Colorimetric Sensing of Silver (I) and Mercury (II) Ions Based on an Assembly of Tween 20-Stabilized Gold Nanoparticles. Anal. Chem., 82 (16), 6830–6837.

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