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SOB Broth 100 g


SOB Broth

SOB Broth (Hanahan’s Broth) is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells. Add 28 grams of Broth in l liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes. Soluble in water.


    • Casein Enzymic Hydrolysate:     20 g/l
    • Magnesium Sulfate: 2.4 g/l
    • Yeast Extract: 5 g/l
    • Potassium Chloride: 0.186 g/l
    • Sodium Chloride: 0.5 g/l
    • Final pH (25°C): 7.0+0.2

Store at room temperature

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