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SOB Broth
SOB Broth (Hanahan’s Broth) is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells. Add 28 grams of Broth in l liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes. Soluble in water.
Contents:
| • Casein Enzymic Hydrolysate: | 20 g/l |
| • Magnesium Sulfate: | 2.4 g/l |
| • Yeast Extract: | 5 g/l |
| • Potassium Chloride: | 0.186 g/l |
| • Sodium Chloride: | 0.5 g/l |
| • Final pH (25°C): | 7.0+0.2 |
Store at room temperature
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