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For complete media, add this amino acid mixture to YNB+Nitrogen+Sugar (dropout base) at 2000.6 mg/L. Filter sterilize or autoclave at 121°C for 15 minutes. To prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.
CONTENTS
| Component | Concentration |
|---|---|
| Adenine | 21 mg/L |
| L-Alanine | 85.6 mg/L |
| L-Arginine | 85.6 mg/L |
| L-Asparagine | 85.6 mg/L |
| L-Aspartic Acid | 85.6 mg/L |
| L-Cysteine | 85.6 mg/L |
| L-Glutamine | 85.6 mg/L |
| L-Glutamic Acid | 85.6 mg/L |
| L-Glycine | 85.6 mg/L |
| L-Histidine | 85.6 mg/L |
| Inositol | 85.6 mg/L |
| L-Isoleucine | 85.6 mg/L |
| L-Leucine | 173.4 mg/L |
| L-Lysine | 85.6 mg/L |
| L-Methionine | 85.6 mg/L |
| Para-Amino Benzoic Acid (PABA) | 8.6 mg/L |
| L-Phenylalenine | 85.6 mg/L |
| L-Proline | 85.6 mg/L |
| L-Serine | 85.5 mg/L |
| L-Threonine | 85.6 mg/L |
| L-Tryptophan | 85.6 mg/L |
| L-Tyrosine | 85.6 mg/L |
| Uracil | 85.6 mg/L |
| L-Valine | 85.6 mg/L |
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