Protein Ladder

(Protein Marker; Prestained Protein Marker)

This protein marker is prestained and designed for monitoring protein separation during SDS-PAGE verification of western transfer efficiency and approximate sizing of proteins.

• Fast - no need for boiling, dilution or the addition of a reducing agent
• Complete - protein marker consists of 11 clearly identifiable bands

Contents: Approximately 0.1~0.4 mg/ml of each protein in the bufffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Note: Second Quantity should not be changed - please contact us for orders more than 6x500 (3 ml total)

Free Shipping within the Continental USA

Protein Ladder 5-245 kDa

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Western transferring. 
• Apply more for thicker (> 1.5 mm) or larger gel. 

The BLUEstain^TM 2 Protein ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 

Protein Ladder 11-175 kDa

Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye

The PiNK Plus Prestained Protein Ladder contains 11 prestained proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

Applications:
• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis. 
• Monitoring of protein transfer onto membranes during Western blotting. 
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.

Shipped in 250 ul vials

Protein Ladder 10-180 kDa

BLUEstain^TM 3 prestained protein ladder is a three-color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 

The BLUEstain^TM 3 prestained protein ladder is designed for monitoring proteins separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. 

Protein Ladder 10-245 kDa

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (> 1.5 mm) or larger gel. 

The BLUEstain^TM Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUEstain^TM Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. 

Recommended Usage

1. Thaw the ladder either at room temperature or at 37-40 °C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel: - 5 μl per well for mini-gels, 2.5 μl per well for blots - 10 μl per well for large gels, 5 μl per well for blots

Store at -20°C for 24 months 
Stable at +4°C for 3 months