Protease Recombinant is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well. The Recombinant Protease is purified by proprietary chromatographic techniques.
Source: Escherichia Coli.
Physical Appearance: Sterile filtered colorless solution.
Cleavage Buffer: 50mM Tris-HCl, pH-7.0 (at 25°C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5°C prior to use.
Storage:
Protease Recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
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EMD Millipore | 324708-2UG |
Promega | V1881 |
TCI America | P0538-25G |
Fisher Scientific | 90053 |