Polypeptone Peptone

Intended Use

Polypeptone Peptone is used as a component in microbiological culture media.

Summary and Explanation

Researchers have found that Polypeptone Peptone meets the nutritional requirements of various bacteria, fungi and mammalian cells, where a single source of casein meat peptone has been unsatisfactory. Polypeptone Peptone has been utilized in culture media for the production of trypsin inhibitor by Cephalosporium sp.,¹in the production of bacterial cellulose by Acetobacter sp. A9² and in the production of succinic acid from whey by Anaerobiospirillum succiniciproducens.³ In addition, Polypeptone Peptone has been used in the mass production of luciferase-bacterial magnetic particles by recombinant Magnetospirillum magneticum AMB-1⁴ and the production of a novel tumor-killing factor by human macrophage-monocyte hybridomas.⁵
Media formulations containing Polypeptone Peptone are specified in standard methods for various applications.^6-11

Principles of the Procedure

Polypeptone Peptone is a mixture of peptones made up of equal parts of pancreatic digest of casein and peptic digest of animal tissue. Polypeptone Peptone includes the high content of amino acids and small polypeptides characteristic of pancreatic digest of casein and the larger polypeptides characteristic of peptic digest of animal tissue. Polypeptone Peptone provides nitrogen, amino acids and vitamins in microbiological culture media.

User Quality Control

Identity Specifications
Polypeptone Peptone
Dehydrated Appearance: Light to dark, yellow to tan, fine, homogeneous,
                                    free of extraneous material.
Solution:                       2.0% solution, soluble in purified water. Solution
                                    is light to medium, yellow to tan, clear to slightly hazy.
Reaction of 2.0%
Solution at 25°C:            pH 6.8-7.5
Cultural Response
Polypeptone Peptone
Prepare a sterile solution of peptone agar without (plain) and with 5% sheep blood (SB) using 10 g of Polypeptone Peptone, 2.5 g of sodium chloride and 6.5 g of agar in 500 mL of purified water. Adjust final pH to 7.2-7.4. Inoculate and incubate plates at 35 ± 2°C for 2-3 days (incubate streptococci with 3-5% CO²).

Typical Analysis

Refer to Product Tables in the Reference Guide section of this manual.

Directions for Preparation from Dehydrated Product

Refer to the final concentration of Polypeptone Peptone in the formula of the medium being prepared. Add product as required.

Procedure

See appropriate references for specific procedures using Polypeptone Peptone.

Expected Results

Refer to appropriate references and procedures for results.

Limitation of the Procedure

1. Do not add Indole Reagent Kovacs until the final lysine deaminase, lysine decarboxylase and motility results have been interpreted.
2. Occasionally, the indole test produces false-negative or falsely weak reactions.⁴

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