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Acidicase Peptone 1 lb


Acidicase Peptone

Intended Use

Acidicase Peptone is used as a nutritional supplement in vitamin assay, susceptibility testing and other laboratory media and microbial fermentation where the high salt content will not interfere.

Summary and Explanation

Acidicase Peptone is a hydrochloric acid hydrolysate of casein. The manufacturing process produces a casein hydrolysate that has a high salt content of approximately 37% and nitrogen content of approximately 8%. The hydrolysis of the casein, a milk protein rich in amino acid nitrogen, is carried out until all the nitrogen is converted to amino acids or other compounds of relative simplicity. It is deficient in cystine, because casein contains little cystine, and in tryptophan, which is destroyed by the acid treatment.

Principles of the Procedure

Acidicase Peptone are acid hydrolyzed casein. Casein is milk protein and a rich source of amino acid nitrogen. Bacto Casamino Acids, Bacto Casamino Acids, Technical, Casamino Acids, Vitamin Assay and Acidicase Peptone provide nitrogen, vitamins, carbon and amino acids in microbiological culture media. Although Bacto Casamino Acids, Bacto Casamino Acids, Technical, Casamino Acids, Vitamin Assay and Acidicase Peptone are added to media primarily because of their organic nitrogen and growth factor components, their inorganic components also play a vital role.17

User Quality Control

NOTE: Differences in the Identity Specifications and Cultural Response testing for media offered as both Difco™ and BBL™ brands may reflect differences in the development and testing of media for industrial and clinical applications, per the referenced publications.
Identity Specifications
BBL™ Acidicase Peptone
Dehydrated Appearance: Fine, homogeneous, free of extraneous material.
Solution:                       2.0% solution, soluble in purified water. Solution
                                   is clear to slightly hazy.
Reaction of 2.0%
Solution at 25°C:           pH 6.5-7.5
Cultural Response
BBL™ Acidicase Peptone
Prepare a sterile solution of 10.0 g of Acidicase Peptone, 2.5 g of sodium chloride and 6.5 g of agar in 500 mL of purified water. Adjust final pH to 7.2-7.4. Inoculate and incubate plates at 35 ± 2°C for 2-3 days under appropriate atmospheric conditions.

Escherichia coli 25922 103-104 Good
Pseudomonas aeruginosa 27853 103-104 Good
Staphylococcus aureus 25923 103-104 Good

Typical Analysis

BRefer to Product Tables in the Reference Guide section of this manual.

Directions for Preparation from Dehydrated Product

Refer to the final concentration Acidicase Peptone in the formula of the medium being prepared. Add appropriate product as required.


See appropriate references for specific procedures using these hydrolysates.

Expected Results

Refer to appropriate references and procedures for results.

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Celebrity Endorsements

1. Mueller and Miller. 1941. J. Immunol. 50:21.

2. Van Niel and Hahn-Hägerdal. 1999. Appl. Microbiol. Biotechnol. 52:617.

3. Takahashi, Sato and Yamada. 2000. J. Bacteriol. 182:4704.

4. Attwood, Klieve, Ouwerkerk and Patel. 1998. Appl. Environ. Microbiol. 64:1796.

5. Strauch and Ehlers. 2000. Appl. Microbiol. Biotechnol. 54:9.

6. Mueller and Hinton. 1941. Proc. Soc. Exp. Biol. Med. 48:330.

7. Levin. 1943. J. Bacteriol. 46:233.

8. Wolf. 1945. J. Bacteriol. 49:463.

9. Nolan. 1971. Mycol. 63:1231.

10. Sarett. 1947. J. Biol. Chem. 171:265.

11. United States Pharmacopeial Convention. 2008. The United States pharmacopeia, 31/The national formulary 26, Supp. 1, 8-1-08, online. United States Pharmacopeial Convention, Inc., Rockville, Md.

12. Downs and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.

13. Horwitz (ed.). 2007. Official methods of analysis of AOAC International, 18th ed., online. AOAC International, Gaithersburg, Md.

14. U.S. Food and Drug Administration. 2001. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md.

15. U.S. Department of Agriculture. Microbiology laboratory guidebook, online. Food Safety and Inspection Service, USDA, Washington, D.C.

16. Eaton, Rice and Baird (ed.). 2005. Standard methods for the examination of water and wastewater, 21st ed. online. American Public Health Association, Washington, D.C.

17. Nolan and Nolan. 1972. Appl. Microbiol. 24:290.

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