(6PGL, 6-Phosphogluconolactonase.)
PGLS is an enzyme in the second step pentose phosphate pathway. 6-Phosphogluconolactonase is crucial for the synthesis of nucleotide sugars and NADPH, the key cause for decreasing power. PGLS transforms 6-phosphogluconolactone to 6-phosphogluconate.
PGLS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 278 amino acids (1-258a.a.) and having a molecular mass of 29.7kDa. PGLS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered clear solution.
Formulation: The PGLS protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 100mM NaCl and 10% glycerol.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Free Shipping within the Continental USA