Luminol, sodium salt

(5-Amino-2,3-dihydro-1,4-phthalazinedione sodium salt; 3-Amino-phthalhydrazide sodium salt)

Yellow to pale green powder
Forms a yellowish, clear solution when dissolved 1% in water.

Horseradish peroxidase, a common marker enzyme for antibodies, can catalyse the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive ELISA assays. Luminescence can be detected at very low levels using extremely sensitive scintillation counters. Luminol is also used for the detection of blood stains in criminal investigation. For this luminol is mixed with a liquid containing hydrogen peroxide. If the mixture comes in contact with hemoglobin, the iron in the hemoglobin accelerates chemiluminescent oxidation of luminol.

Protect from air.

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