KITLG, Human

(Recombinant Human KIT Ligand, KitI, KL-1, Stem Cell Factor, SCF Mast Cell Growth Factor, MGF)

Purity: >97% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

C-kit ligand, the recently identified ligand for the kit tyrosine kinase receptor, is mapped to the mouse S1 locus. This pleiotropic cytokine, alternately known as stem cell factor (SCF), mast cell growth factor (MGF) and steel-factor (SLF), plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. In vitro and in vivo, KITLG can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. KITLG acts in a synergistic manner on purified primitive human and mouse hematopoietic precursors, with various growth factors (such as IL1, IL3, IL6, IL7, and Epo), to induce myeloid, erythroid and lymphoid lineage colony formation. The finding that KITLG is also expressed in the nervous system suggests a possible role for KITLG in the development of the nervous system.

rHuKITLG General Information
Amino Acid Sequence (164 aa):
EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum, and 1 µg/ml heparin sulfate.

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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