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Kanamycin Monosulfate 5 g


Kanamycin Monosulfate

Molecular Biology Grade

  CAS Number:
  Chemical Formula:
  C18H36N4O11 H2SO4
  Molecular Weight:
  582.6 g/mol

Approximately 800 ug/mg
Kanamycin B: 0.82%

Kanamycin is an antibiotic typically used against prokaryotes. It is an aminoglycoside that indirectly interrupts protein synthesis in non-resistant cells by binding to the ribosome and disrupting translocation of tRNA during translation.

Aminoglycosides like kanamycin "irreversibly" bind to specific 30S-subunit proteins and 16S rRNA. Specifically, kanamycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes.

Store dessicated at -20°C
Soluble in water

Free Shipping within the Continental USA

Protocol Using Kanamycin:

How to make a 50 mg/ml Kanamycin Stock Solution

View this method on protocols.io and use it directly at your bench

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Celebrity Endorsements

Kowalinski, E.; et. al. (2007) Expression, purification, crystallization and preliminary crystallographic analysis of the proliferation-associated protein Ebp1. Acta Crystallographica Section F. F6., 768-770.

- The paper describes their cell expression. The method and concentration of kanamycin used has become fairly standardized. In this case, cells were grown at 310 K in lysogeny broth medium complemented with 50 mg/ml kanamycin.

Klink, B.u.; Scheidig, A.J. (2010) New insight into the dynamic properties and the active site architecture of H-Ras p 21 revealed by X-ray crystallography at very high resolution. BMC Structural Biology. 10:38.

Cells were grown in 10 l Standard I-medium (50 μg/ml ampicillin, 50 μg/ml kanamycin) at 37°C.

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