FGF18, Murine

(Recombinant Murine Fibroblast Growth Factor 18, FGF-18)

Purity: >95% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

FGF18 is a member of the Fibroblast Growth Factor family of proteins. FGF18 has been shown in vitro to induce neurite outgrowth in PC12 cells.

Studies of the similar proteins in mouse and chick suggest that this protein is a pleiotropic growth factor that stimulates proliferation in tissues such as the liver and small intestine. FGF18 acts as a trophic factor for elastic chondrocytes in vivo and articular chondrocytes cultured in vitro and plays an autocrine role in normal articular cartilage repair.

FGF18 –/– mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF18 has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. It is most closely related to FGF8 and FGF17.

rMuFGF18 General Information
Amino Acid Sequence (180 aa):
EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SRRIRPTHPG

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1 - 1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2 - 8°C. Stock solutions should be aliquoted and stored at - 80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum, and 1 μg/ml heparin sulfate..

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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