Carbenicillin Disodium Salt

Molecular Biology Grade

Carbenicillin an analog to ampicillin is an inhibitor of bacterial cell wall synthesis by disrupting peptidoglycan cross-linking.  Specifically, transpeptidases is inactivated within the cytosollic of the bacterial cell membrane.  High activity against gram-negative bacteria. Commonly used for the elimination of Agrobacterium species after inoculation. B-lactamase sensitive.

Long Term Storage dessicated at -20°C
Soluble in water

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Preparation of 50 mg/ml Carbenicillin Stock Solution

(Applicable for Salmonella typhimurium or E. coli)

1. Take 50 mg of Carbenicillin and add 1 ml of molecular biology grade water to get a final concentration of 50 mg/ml.
2. Use a vortex to dissolve.
3. Draw solution into 1 ml syringe.
4. Pass solution through a 0.22-micron filter into a fresh sterile container.
5. Store at -20 °C
6. When ready to use add 2 μl of stock solution per 1 ml of media to get a final concentration of 2 μl/ml.

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