BMP4, Human Inactive Protein

(Recombinant Human Bone Morphogenetic Protein 4, Inactive Protein BMP4, BMP2b, BMP2b1, BMP2b-1)

Purity: >95% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

Bone Morphogenetic Proteins (BMPs) belong to the TGF-ß (Transforming Growth Factor) superfamily of structurally related signaling proteins.

BMP4 is one of at least 15 structurally and functionally related BMP’s, originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMP’s have been shown to regulate the growth, differentiation, chemotaxis and apoptosis of a variety of cell types; including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. The active form of BMP4 can consist of a dimer of two identical proteins or a heterodimer of two related BMPs. BMP4 is essential for various processes in early development, beginning with gastrulation and mesoderm formation.

BMP4 Inactive protein is an excellent molecular marker for Western Blotting and ELISA or as the molecular standard in detecting secreted BMP4 in reduced SDS-Page gels. It can also be used for preparing antibodies for the BMP4 monomer.

rHuBMP4 General Information
Amino Acid Sequence (117 aa):
MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C Further dilutions should be made in appropriate buffered solutions.

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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