(5-Bromo-3-indoxyl-β-D-galactopyranoside; Blue-Gal; Lapis™-β-D-Gal)
Purity > 99.0 %
CAS Number: |
97753-82-7
|
Chemical Formula: |
C14H16BrNO6
|
Molecular Weight: | 374.2 g/mol |
Bluo-gal is used as an alternative to X-gal that produces a darker blue color, which is ideal for white/blue assay screening. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing bluo-gal.
Soluble in dimethylformamide (DMFA) and dimethyl sulfoxide (DMSO)
Store at -15°C, protect from light.
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Alfa Aesar | J65978-MC |
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TCI America | B3470-20MG |
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