Bacterial Protease Inhibitor (100x)

Bacterial protease inhibitor cocktail contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of ProBlock™ Gold Bacterial inhibits over 90% of protease activities.

Optional EDTA is provided to inhibit metalloproteases. Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of EDTA may be detrimental to the protein’s activity. Furthermore, EDTA inhibits the purification of proteins by immobilized metal affinity chromatography. Therefore we provide an optional EDTA solution.

Benefits:

• Greater Range of Inhibition – 95%+
• Better than tablets – the ready-to-use, refrigerator-stable, 100X liquid format is more effective and easier to use than traditional tablet products; just pipette exactly the amount you need!
• No proprietary ingredients – fully disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A, and PMSF
• Multiple package sizes 
• Two popular formulations – regular formulation includes a separate vial of EDTA solution for optional metallo-protease inhibition; EDTA-free formulation ensures compatibility with isoelectric focusing
• Specifically optimized for mammalian proteases

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