Clone | PAX-14 |
Size | 100μg/vial |
Form | lyophilized |
Ig type | mouse IgG1 |
Immunogen | C-terminal part of recombinant chicken paxillin (amino acids 305-559). |
Application | WB; ICC |
Cross Reactivity | Human, mouse, rat, chicken. |
Reconstitution | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, |
the antibody concentration will be 100μg/ml. | |
Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
It can also be aliquotted and stored frozen at -20˚C for a longer time. | |
Avoid repeated freezing and thawing. | |
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. |