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Anti-NME2 Polyclonal 100ug

Price:
$215.00
SKU:
BI-PA1830-100UG
Availability:
Usually Ships within 24 hrs.
Quantity:

Anti-NME2 Polyclonal

Size  100μg/vial
Form Lyophilized
Ig type Rabbit IgG
Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human NME2, different from the related mouse and rat sequences by one amino acid.
Application WB,IHC-P,ICC
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. 
             It can also be aliquotted and stored frozen at -20˚C for a longer time. 
  Avoid repeated freezing and thawing.

NME2(NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein (NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Little to no expression was detected in other mouse tissues examined. Using Northern blot analysis, Masse et al. (2002) detected high expression of mouse Nme2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous Nme2 expression. The mouse and human NME2 genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al. (2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NDPBK is critical to the channel activity and the activation of CD4 T cells.


 

Concentration

Tested Species

Concluded Species

Antigen Retrieval

WB

0.1-0.5μg/ml

Hu, Rat

Ms

-

IHC-P

0.5-1μg/ml

Hu, Rat, Ms

-

By Heat

ICC

0.5-1μg/ml

Hu

-

-


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