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Categories
Anti-mouse TNFα Polyclonal
| Size | 100μg/vial |
| Form | lyophilized |
| Ig type | rabbit IgG |
| Immunogen | E. coli-derived mouse TNFα recombinant protein (Position: L80-L235). |
| Application | WB;IHC-P;ELISA;Neu;IP |
| Reconstitution | 0.2ml of distilled water will yield a concentration of 500μg/ml. |
| Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
| It can also be aliquotted and stored frozen at -20˚C for a longer | |
| time. Avoid repeated freezing and thawing. | |
|
Tumor necrosis factor-alpha (TNFA), also known as TNFR1, is a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Mouse TNFα shares 95% amino acid sequence homology with rat. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. TNFR1 maps to 12p13. TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival. |
|
|
Recommended Concentration |
Species |
Antigen Retrieval |
|
|
WB |
0.1-0.5μg/ml |
Mouse |
- |
|
IHC-P |
0.5-1μg/ml |
Mouse |
By Heat |
|
ELISA |
0.1-0.5μg/ml |
Mouse |
- |
|
Neu |
√ |
Mouse |
- |
|
IP |
√ |
Mouse |
- |
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