Size | 100μg/vial |
Form | Lyophilized |
Ig type | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human ALOX5, different from the related rat and mouse sequences by two amino acids. |
Application | WB,IHC-P |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
It can also be aliquotted and stored frozen at -20˚C for a longer time. | |
Avoid repeated freezing and thawing. | |
ALOX5(ARACHIDONATE 5-LIPOXYGENASE),also known as LOG5 or 5-LO(5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme. |
Concentration |
Tested Species |
Concluded Species |
Antigen Retrieval |
|
WB |
0.1-0.5μg/ml |
Hu |
- |
- |
IHC-P |
0.5-1μg/ml |
Hu |
- |
By Heat |