Size | 100μg/vial |
Form | lyophilized |
Ig type | rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human CACYBP, identical to the related rat and mouse sequences. |
Application | WB; IHC-P; ICC |
Cross Reactivity | Human, rat. |
Reconstitution | 0.2ml of distilled water will yield a conncentration of 500μg/ml. |
Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
It can also be aliquotted and stored frozen at -20˚C for a longer | |
time. Avoid repeated freezing and thawing. | |
CACYBP(Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function. |