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aFGF (FGF1), human 10ug

Price:
$53.00
SKU:
GB-a0101-10
Quantity:

aFGF, human

(Recombinant Human Fibroblast Growth Factor 1, FGF1, aFGF, Acidic FGF, Endothelial cell growth factor)

Purity: >95% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

FGF1 lacks signal peptides and is believed to be secreted by a non-classical mechanism. It has been detected in large amounts in the brain and is expressed in hepatocyte cells, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelial cells and pituitary basophils and acidophils

FGF1 exhibits considerable species cross-reactivity and stimulates the proliferation of cells of mesodermal origin as well as many cells of neuroectodermal, ectodermal, and endodermal origin. It is most closely associated with FGF2 (bFGF).

FGF1 is a member of the Fibroblast Growth Factor family of proteins.

HuFGF1 General Information
Amino Acid Sequence (141aa):
MFNLPPGNYK KPKLLYCSNG GHFL
RILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D


Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum, and 1 µg/ml heparin sulfate.


Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

 

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