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DTT 25 g

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(DTT; (D,L)-1,4-Dithiothreitol; DL-Dithiothreitol; Cleland's Reagent; Cleland Reagent; threo-2,3-dihydroxy-1,4-dithiolbutane)

Purity > 99.5 %

Molecular Biology Grade

  CAS Number:   3483-12-3
  Chemical Formula:   C4H10O2S2
  Molecular Weight:   154.25 g/mol


Dithiothreitol is a commonly used reducing agent and is soluble in a range of solvents including water, ethanol, acetone and ethyl acetate.  DTT is quite hygroscpoic and in some applications has been replaced by TCEP HCl. The use of dithiothreitol (DTT) was popularized by William Wallace Cleland who first published on its reducing potential in 1964.  This led to dithiothreitol being referred to as Cleland's reagent. DTT has a reducing potential of -0.33 V at pH 7.  DTT should only be used as a reducing agent above a pH 7 since the thiol group needs to deprotonated becoming a thiolate (S-) species.  The thiolate group is responsible for breaking apart the disulfide bonds within proteins.

DTT is listed under two CAS numbers 27565-41-9 and 3483-12-3.

Store: -20 °C

Free within the Continental USA

Dithiothreitol Certification of Analysis

Manufacture and Expiration Data available upon request.

  Test   Speficiations   Results
  Appearance   White crystalline powder   Corresponds
  Absorbance (280 nm)   Colorless, clear   0.1 Molar
  % Oxidized DTT    0.3 %   Conforms
  Melting Point   40-44 °C   42 °C
  pH 0.1 M   N/A   4-6
  FTIR   Reference spectrum   Corresponds


Preparation of 1 M Dithiothreitol (DTT) Stock Solution

  1. Dissolve 15.45 g of dithiothreitol in 100 mL of water to obtain a final concentration of 1 M (154.5 mg/mL).
  2. Aliquot into 1.6 mL tubes and store the tubes at -20 °C.

View this method on protocols.io and use it directly at your bench

Celebrity Endorsements

Cleland, W.W. (1964) Dithiothreitol, a New Protective Reagent for SH Groups. Biochemistry. 480-482.

Wang, H.; Qi, M.; Cutler, A.J. (1993) A simple method of preparing plant samples for PCR. Nucleic Acids Research, Vol. 21, 4153-4154.

The standard Taq PCR buffer (1 x, consisting of 10 mM Tris, 50 mM KCl, 1.5 mM MgCl2, 0.01% gelatin and 3 mM DTT (dithiothreitol) with pH 8.3) were shown to produce good results. In regards to standard PCR buffer individual components of the following were explored: Tris (100 mm, pH 8.3) or KCI (500 mM) or MgCl2 (15 mM) or DTT (30 mM). Tris proved to be the most critical.

References Citing this Product:
Guo, L.W. (2007). Protein Expression and Purification, 51(2), 187-197.

Tan A.; et al. (2008). Energetics of OCP1–OCP2 complex formation. Biophysical Chemistry, 134 (1-2). 64-71.

Tan, A.; Henzl, M.T. (2009) Conformational stabilities of guinea pig OCP1 and OCP2. Biophysical Chemistry, 144. 3. 108-118.

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