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X-gluc
(5-Bromo-4-chloro-3-indolyl-b-d-glucuronic acid; 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt)
Purity: > 99.0% (Molecular Biology Grade)
| CAS Number: |
114162-64-0
|
| Chemical Formula: | C14H13BrClNO7·C6H13N |
| Molecular Weight: |
521.79 g/mol
|
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.
Protocol Using X-Gluc
How to make a 20 mg/mL X-Gluc Stock Solution
View this method on protocols.io and use it directly at your bench
Comparable Items:
| Acros Organics | 33119-1000 |
| Alfa Aesar | B21195-MF |
| Alfa Aesar | B21195-MA |
| TCI America | B3620-100MG |
| TCI America | B3620-10MG |
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