Tomato Juice Broth

Intended Use

Tomato Juice Broth is used for cultivating yeasts and other aciduric microorganisms.

Summary and Explanation

In 1925, Mickle and Breed¹ reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp² investigated the use of tomato juice on bacterial development and found that the growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White’s³ modification, is especially useful in cultivating L. acidophilus from clinical specimens and foodstuffs.⁴
Tomato Juice Broth is recommended for use in cultivating and isolating yeasts, lactobacilli and other aciduric microorganisms from clinical specimens and foods.

Principles of the Procedure

Tomato Juice Broth
Tomato juice is a source of carbon, protein and nutrients. Yeast extract is a source of trace elements, vitamins and amino acids. Dipotassium phosphate and monopotassium phosphate provide buffering capability. Magnesium sulfate, ferrous sulfate and manganese sulfate provide inorganic ions. Sodium chloride is a source of essential ions.

User Quality Control

Identity Specifications
Tomato Juice Broth
Dehydrated Appearance: Tan, free-flowing, homogeneous and may
                                      contain dark particles.
Solution:                         4.1% solution, soluble in purified water
                                      upon boiling. Solution is dark amber, clear.
Prepared Appearance:      Dark amber, clear.
Reaction of 4.1%
Solution at 25°C:             pH 6.7 ± 0.2>
Cultural Response
Tomato Juice Broth
Prepare the medium per label directions. Inoculate and incubate at 35 ± 2°C for 18-72 hours.

Formula

Tomato Juice Broth
Approximate Formula* Per Liter
Tomato Juice (from 400 mL)........................................ 20.0 g
Yeast Extract.............................................................. 10.0 g
Dextrose.................................................................... 10.0 g
Dipotassium Phosphate.................................................. 0.5 g
Monopotassium Phosphate............................................. 0.5 g
Magnesium Sulfate........................................................ 0.1 g
Sodium Chloride.......................................................... 0.01 g
Ferrous Sulfate............................................................ 0.01 g
Manganese Sulfate....................................................... 0.01 g
*Adjusted and/or supplemented as required to meet performance criteria.

Directions for Preparation from Dehydrated Product

Equilibrate the medium to room temperature before opening.
1. Suspend the powder in 1 L of purified water:
    Tomato Juice Broth – 41 g.
    Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
3. Autoclave at 121°C for 15 minutes. Avoid overheating Tomato Juice Agar Special, which could cause a softer medium.
4. Test samples of the finished product for performance using stable, typical control cultures.

Procedure

See appropriate references for specific procedures.

Expected Results

Refer to appropriate references and procedures for results.

*Store at 2-8° C.

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