TNF-α (His), Human

(Recombinant Human Tumor Necrosis Factor, His TNF-α, TNF-alpha, Cachectin, Cachexin)

Purity: >97% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

Tumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF is glycosylated, but non-glycosylated recombinant TNF has comparable biological activity. The biologically active native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is crossreactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types. TNF-α, His has an identical amino acid sequence to Human TNF-α, but with 6x His at the N-terminus for protein purification.

rHuTNF-His General Information
Amino Acid Sequence (157 aa plus 6x His at N-terminus):
MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD AESGQVYFG IIAL

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 10mM HAc to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions..

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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