Taq DNA Polymerase

(Taq Polmerase)

Taq Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has both a 5'->3' DNA polymerase and a 5'->3' exonuclease activity but lacks a 3'-> 5' exonuclease activity.  The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.  Taq is commonly used for the PCR amplification of DNA templates.

A unit of Taq incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C. Unit assay conditions: 25 mM TAPS (pH 9.3), 50 mM KCl, 2 mM MgCl₂, 1 mM DTT, 0.5 mg/ml activated salmon sperm DNA, 0.2 mM dATP, dCTP, dGTP and dTTP.

Store at -20° C.

Recommended Usage:

Usually 1-2.5u of Taq DNA Polymerase are used in 100 μl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products.  However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA Polymerase (2-3u) are helpful in obtaining a better yield of amplification products.

Taq Formulation:

Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.

Technical Information:

Contents:

10X Reaction Buffer without MgCl2

Separate 25mM MgCl2 Solution.

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