Soytone Culture Media

Intended Use

Soytone are used in media for the cultivation of a large variety of organisms, including fungi.

Summary and Explanation

Select Soytone demonstrates excellent growth support for Escherichia coli. Select Soytone is also used in molecular genetics media such as Select APS™ Super Broth. Subtle differences in the hydrolysation process give Select Soytone improved performance in cell culture.
Soytone was found to be effective in the recovery of stressed E. coli.2 It was found that Bacto Soytone with the addition of 7 vitamins replaced yeast extract as an economical alternative for the production of lactic acid by Lactobacillus rhamnosus.3 It should be noted that Bacto Soytone utilizes an enzyme in the digestion of the soy flour that has been sourced from animal.
Media formulations containing Phytone Peptone or Bacto Soytone are specified in various standard methods for multiple applications.4-8

Principles of the Procedure

Select Soytone and Bacto Soytone are enzymatic digests of soybean meal. The nitrogen source in the soy peptones contains naturally occurring high concentrations of vitamins and carbohydrates of soybean.

Identity Specifications
Select Soytone
Dehydrated Appearance: Tan, free-flowing, homogeneous powder.
Solution:                       2.0% solution, soluble in purified water. Solution is clear to moderately hazy.
Reaction of 2.0%
Solution at 25°C:           pH 6.5-7.5
Bacto Soytone
Dehydrated Appearance: Light to medium tan, free-flowing, homogeneous.
Solution:                       2.0% solution, soluble in purified water. Solution is light to medium amber,
                                   clear to very slightly hazy. A small amount of precipitate is acceptable.
Reaction of 2.0%
Solution at 25°C:           pH 6.5-7.5
Cultural Response
Biochemical Reactions
Bacto Soytone
Prepare sterile solutions of Bacto Soytone as directed below. Adjust final pH to 7.2-7.4. Inoculate and incubate at 35 ± 2°C for 18-48 hours.

Growth Response
Bacto Soytone
Prepare a sterile solution with 2% Bacto Soytone, 0.5% sodium chloride and 1.5% agar. Adjust final pH to 7.2-7.4. Inoculate and incubate plates at 35 ± 2°C for 18-48 hours.

Typical Analysis

Refer to Product Tables in the Reference Guide section of this manual.

Directions for Preparation from Dehydrated Product

Refer to the final concentration of Select Soytone and Bacto Soytone in the formula of the medium being prepared. Add appropriate product as required.

Procedure

See appropriate references for specific procedures using Select Soytone and Bacto Soytone

Expected Results

Refer to appropriate references and procedures for results.

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