ISP Medium 1

Intended Use

ISP Medium 1, ISP Medium 2 and ISP Medium 4 are used for characterizing Streptomyces species according to the International Streptomyces Project (ISP).¹

Summary and Explanation

ISP media were developed by Difco Laboratories for the International Streptomyces Project (ISP) in order to select stable properties and reproducible procedures for characterization of Streptomyces species.¹
ISP Medium 1 is also referred to as Tryptone-Yeast Extract Broth.

Principles of the Procedure

Peptone and yeast extract provide nitrogen, vitamins, carbon and amino acids in ISP Medium 1.

User Quality Control

Identity Specifications
ISP Medium 1
Dehydrated Appearance: Beige, free-flowing, homogeneous.
Solution:                        0.8% solution, soluble in purified water upon
                                     boiling. Solution is light amber, clear to very
                                     slightly opalescent.
Prepared Appearance:     Light amber, clear to very slightly opalescent.
Reaction of 0.8%
Solution at 25°C:             pH 7.0 ± 0.2
Cultural Response
ISP Medium 1
Prepare the medium per label directions. Inoculate tubes of prepared ISP Medium 1 with the test organisms and incubate at 30 ± 2°C for up to 96 hours. 

Formula

ISP Medium 1
Approximate Formula* Per Liter
Pancreatic Digest of Casein........................................... 5.0 g
Yeast Extract................................................................ 3.0 g
*Adjusted and/or supplemented as required to meet performance criteria.

Directions for Preparation from Dehydrated Product

1. Suspend the powder in 1 L of purified water:
    ISP Medium 1 – 8 g;
    Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
3. Autoclave at 121°C for 15 minutes.
4. Mix thoroughly while dispensing ISP Medium 4.
5. Test samples of the finished product for performance using stable, typical control cultures.

Procedure

For details on the use of these media for characterization of Streptomyces species, consult the reference.¹ For a complete discussion on the isolation and maintenance of Streptomyces species refer to appropriate references.^2,3

Expected Results

Refer to appropriate references and procedures for results.

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