(Heat shock 27kDa protein 2, DMPK-binding protein, MKBP, HSP27, LOH11CR1K.)
Heat shock protein beta-2 (HSPB2) is a member of the small heat shock protein (HSP20) family. HSPB2 is a regulator of DMPK kinase.
HSPB2 Human Recombinant produced in E. coli is a single polypeptide chain containing 206 amino acids (1-182) and having a molecular mass of 22.8kDa. HSPB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: Greater than 90% as determined by SDS-PAGE.
Source: E.coli.
Physical Appearance: Sterile Filtered colorless solution.
Formulation: The HSPB2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol.
Amino acid sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMSGRSV PHAHPATAEY EFANPSRLGE QRFGEGLLPE EILTPTLYHG YYVRPRAAPA GEGSRAGASE LRLSEGKFQA FLDVSHFTPD EVTVRTVDNL LEVSARHPQR LDRHGFVSRE FCRTYVLPAD VDPWRVRAAL SHDGILNLEA PRGGRHLDTE VNEVYISLLP APPDPEEEEE AAIVEP
Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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