FTA Hemagglutination Buffer

Intended Use

FTA Hemagglutination Buffer (Phosphate Buffered Saline, pH 7.2) is used in the FTA-ABS test and other serological procedures as a diluent and for washing slide preparations.

Summary and Explanation

This phosphate buffered saline product is widely used in immunology as a diluent in serological procedures. It is referred to as FTA Hemagglutination Buffer because of its use in the Fluorescent Treponemal Antibody-Absorption (FTA-ABS) test, which is a serological test for syphilis.

Principles of the Procedure

This phosphate buffered saline provides an isotonic environment for microorganisms during manipulation pursuant to the performance of serological test procedures.

User Quality Control

Identity Specifications
FTA Hemagglutination Buffer
Dehydrated Appearance: White, fine, homogeneous, free of extraneous
                                     material.
Solution:                         0.923% solution, soluble in purified water. Solution
                                      is colorless, clear.
Prepared Appearance:      Colorless, clear.
Reaction of 0.923%
Solution at 25°C:             pH 7.2 ± 0.1

Formula

FTA Hemagglutination Buffer
Approximate Formula* Per Liter
Sodium Chloride.......................................................... 7.65 g
Disodium Phosphate.............................................. 1,268.8 mg
Monosodium Phosphate................................................... 0.1 g
Monopotassium Phosphate........................................ 211.3 mg
*Adjusted and/or supplemented as required to meet performance criteria.

Directions for Preparation from Dehydrated Product

1. Dissolve 9.23 g of the powder in 1 L of purified water.
DO NOT AUTOCLAVE.
2. Test samples of the finished product for performance.

Procedure

Refer to specific serological procedures for the use of this buffer.¹

*Store at 2-8° C.

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