DNAse I

(Deoxyribonuclease I; DNAse 1)

Molecular Biology Grade

Deoxyribonuclease I efficiently hydrolyzes single-stranded or double-stranded DNA in the presence of divalent cations, producing 3’- hydroxyl oligonucleotides. Useful in crucial RNA preparations from tissue or bacterial cell cultures. Attention is need to ensure all equipment and glassware is RNase free.

Solution:  Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1mM calcium chloride. Store at -20°C

Lyophilized: Chromatographically purified to remove RNase and protease contents arrives in a vial containing glycine and calcium as stabilizer. Please take care as lypophilized DNAse I is moisture sensitive. Store 2-8°C

RNase and Protease Free

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DNAse Quantity

DNase I Solution ≥2,000 Kunitz units per ml

DNAse I Lyophilized ≥2,000 Kunitz units per mg dry weight