CSF2, Rat

(Recombinant Rat Colony Stimulating Factor 2 (granulocyte-macrophage), CSF2, GM-CSF, GMCSF,, Molgramostin, MGI-1GM (Macrophage granulocyte inducer-1GM)))

Purity: >98% by SDS-PAGE and HPLC analyses

Source: Escherichia coli

Colony Stimulating Factor 2 (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors.

It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. On mature hematopoietic cells, CSF2 is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. CSF2 promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity.

It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and CSF2 transfected tumor cells are utilized as cancer vaccines. Mature rat CSF2 shares 69% with mouse CSF2. Rat CSF2 is fully active on mouse cells, although Mouse CSF2 is only weakly active on rat cells.

rRtCSF2 General Information
Amino Acid Sequence (128 aa):
MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK

Reconstitution:
The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions.

Storage:
The lyophilized sample is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for maximal stability. Avoid repeated freeze/thaw cycles.

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