Cladera, J.; et. al. (1997) Liposome solubilization and membrane protein reconstitution using Chaps and Chapso. European Journal of Biochemistry. Vol. 243. 798-804.

-The paper examines the solubilization of the liposome and reconstitution of two transport proteins using CHAPS and CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-l-propanesulfonate). The solubilization of unilamellar liposomes was followed by absorption experiments and the process was shown to fit well to the three-stage model described by other detergents. The solubilization parameters have been determined and the detergent to phospholipid ratios at which the lamellar-to-micellar transition initiates are described. The use of CHAPSO and CHAPS for membrane protein reconstitution studies yields a reproducible method of achieving active proteoliposomes, homogeneous in size with a low permeability and therefore is suited for transport measurements.