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Casitone
Intended Use
Bacto Casitone, Trypticase Peptone, Bacto Tryptone and BiTek Tryptone are used in preparing microbiological culture media.
Ingredients, where noted, meet United States Pharmacopeia (USP) performance specifications.
Summary and Explanation
The manufacturing process for an enzymatic digest of casein is not as destructive as an acid hydrolysis. Thus, the casein is not broken down as completely into its constituent components. In many cases this makes for a more nutritious hydrolysate, especially for those organisms that prefer peptides to amino acids.
Bacto Casitone can be used as a component in microbiological culture media or in fermentation applications. A recent publication has also reported that the stability of lyophilized influenza virus vaccine was augmented by the addition of 2% Casitone.1
Principles of the Procedure
Bacto Casitone, Trypticase Peptone, Bacto Tryptone and BiTek Tryptone are pancreatic digests of casein. Casein is the main milk protein and a rich source of amino acid nitrogen.
User Quality Control
Identity Specifications
Casitone
Dehydrated Appearance: Tan, free-flowing, granules.
Solution: 1.0%, 2.0% and 10.0 % solutions, soluble in
purified water. 1.0% solution is light amber,
clear. 2.0% solution is light to medium amber,
clear, may have a slight precipitate. 10.0%
solution is medium to dark amber, clear to very
slightly opalescent, may have a precipitate.
Reaction of 1.0%
Solution at 25°C: pH 6.8-7.4
Cultural Response
Biochemical Reactions
Casitone
Prepare a sterile solution as directed below. Adjust final pH to 7.2-7.4. Inoculate and incubate at 35 ± 2°C for 18-48 hours.
| TEST | TEST SOLUTION | ORGANISM | ATCC™ | INOCULUM CFU |
RESULT |
| Fermentable Carbohydrates | 2% | Escherichia coli | 25922 | ~107 | Negative |
| Indole Production | 0.1% | Escherichia coli | 29552 | 0.1 mL, undiluted | Positive |
| Acetylmethylcarbinol Production | 0.1% with 0.5% dextrose | Enterobacter aerogenes | 13048 | 0.1 mL, undiluted | Positive |
| Hydrogen Sulfide Production | 1% | Salmonella enterica subsp. enterica serotype Typhimurium |
14028 | 0.1 mL, undiluted | Positive |
Growth Response
Casitone
Prepare a sterile solution with 2.0% Bacto Casitone, Bacto Tryptone or BiTek Tryptone, 0.5% sodium chloride and 1.5% agar. Adjust final pH to 7.2-7.4. Inoculate and incubate plates at 35 ± 2°C for 18-48 hours.
| ORGANISM | ATCC™ | INOCULUM CFU |
RECOVERY |
| Escherichia coli | 25922 | 30-300 | Good |
| Staphylococcus aureus | 25923 | 30-300 | Good |
Typical Analysis
Refer to Product Tables in the Reference Guide section of this manual.
Directions for Preparation from Dehydrated Product
Refer to the final concentration of Bacto Casitone, Trypticase Peptone, Bacto Tryptone and BiTek Tryptone in the formula of the medium being prepared. Add appropriate product as required.
Procedure
See appropriate references for specific procedures using Bacto Casitone, Trypticase Peptone, Bacto Tryptone and BiTek Tryptone.
Expected Results
Refer to appropriate references and procedures for results.
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Celebrity Endorsements
2. Horowitz (ed.). 2007. Official methods of analysis of AOAC International, 18th ed., online. AOAC International, Gaithersburg, Md.
3. United States Pharmacopeial Convention, Inc. 2008. The United States pharmacopeia 31/The national formulary 26, Supp. 1, 8-1-08, online. United States Pharmacopeial Convention, Inc., Rockville, Md.
4. Takahashi and Yamada. 2000. J. Bacteriol. 182:4704.
5. Nagel, Oostra, Tramper and Rinzema. 1999. Process Biochem. 35: 69.
6. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C. 8. Eaton, Rice and Baird (ed.). 2005. Standard methods for the examination of water and wastewater, 21st ed., online. American Public Health Association, Washington D.C.
9. Wehr and Frank (ed.). 2004. Standard methods for the examination of dairy products, 17th ed. American Public Health Association, Washington D.C.
10. U.S. Environmental Protection Agency. 2000. Improved enumeration methods for the recreational water quality indicators: Enterococci and Escherichia coli. EPA-821/R-97/004. Office of Water, Washington, D.C.
11. U.S. Department of Agriculture. 1998. Microbiology laboratory guidebook, 3rd ed. Food Safety and Inspection Service, USDA, Washington, D.C.
12. Council of Europe. 2008. European pharmacopoeia, 6th ed. Council of Europe, Strasbourg, France.
13. Sivakesavs, Chen, Hackett, Huang, Lam, Lam, Siu, Wong and Wong. 1999. Process Biochem. 34:893.
14. Wen and Chen. 2001. Enzyme Microbia Technol. 29:341. 7. U.S. Food and
Drug Administration. 2001. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md.
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