Brefeldin A

Purity > 99.0%

Brefeldin A (BFA) is a fungal metabolite that reversibly interferes with protein transport from the endoplasmic reticulum to the Golgi apparatus.

Brefeldin A is a lactone antibiotic with a wide range of anti-bacterial activity, however it is mainly used to study protein transport. Treatment with BFA would lead to rapid accumulaztion of proteins in ER, and collapse of the Golgi stacks. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the golgi complex/endoplasmic reticulum.

Optimal conditions for use are cell type and time-dependent.

Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Brefeldin A Solution is supplied as a 1,000X solution and should be diluted to 1X in cell culture medium.

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