Beef Heart for Infusion

Intended Use

Beef Heart for Infusion is used in preparing microbiological culture media.

Summary and Explanation

Beef Heart for Infusion is prepared from fresh beef heart tissue and dried to a powdered form. Beef Heart for Infusion is processed from large quantities of raw material, retaining all the nutritive and growth-stimulating properties of the fresh tissues. One hundred grams of Beef Heart for Infusion are the equivalent of 500 g of fresh heart tissue. Beef Heart for Infusion supplies the nutritional requirements for growth of microorganisms in Heart Infusion media.
One of the first media used for the cultivation of bacteria was a liquid medium containing an infusion of meat. Huntoon1 used fresh beef heart and Bacto Peptone to prepare a “hormone” broth to retain growth-promoting substances. Highly pathogenic organisms, such as meningococci and pneumococci, could be grown on infusion medium without enrichments.¹
Beef Heart for Infusion is a component of Heart Infusion media. Heart Infusion media are used in the mass production of microorganisms for vaccine production and are specified in standard methods for other multiple applications.^2-7

Principles of the Procedure

Beef Heart for Infusion provides nitrogen, amino acids and vitamins in microbiological culture media.

User Quality Control

Identity Specifications
Beef Heart for Infusion
Dehydrated Appearance: Tan to medium brown, fine, homogeneous.
Solution:                        5.0% solution, not completely soluble in purified
                                     water. Solution, after filtration, is light to medium
                                     amber, clear to slightly opalescent, may have a
                                     precipitate.
Reaction of 5.0%
Solution at 25°C:            pH 7.5-7.8
Cultural Response
Beef Heart for Infusion
Prepare a 5% solution of Beef Heart for Infusion. Infuse for one hour at 50 ± 2°C. Heat to boiling for 3-5 minutes and filter. Add 2% Proteose Peptone No. 3, 0.5% sodium chloride and 0.005% dextrose to the filtrate. Adjust pH to 7.5-7.8. Boil and filter before autoclaving. Inoculate and incubate tubes at 35 ± 2°C for 18-48 hours.

Typical Analysis

Refer to Product Tables in the Reference Guide section of this manual.

Directions for Preparation from Dehydrated Product

Infusions can be prepared using 50 g of Beef Heart for Infusion per liter of purified water. For best results, infuse at 50° C for 1 hour. Heat the infusion to boiling for a few minutes to coagulate some of the proteins and filter. Add peptone and remaining ingredients of the medium to the filtrate. Adjust the pH to 7.5-7.8. Boil the medium and filter before autoclaving. Consult appropriate references for further directions on preparation of specific products.

Procedure

See appropriate references for specific procedures using Beef Heart for Infusion.^2-4

Expected Results

Refer to appropriate references and procedures for results.

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