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Categories
Anti-TNF alpha Polyclonal
| Size | 100μg/vial |
| Form | Lyophilized |
| Ig type | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human TNFalpha, different from the related mouse sequence by five amino acids, and rat sequence by seven amino acids. |
| Application | WB,IHC-P |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Storage | At -20˚C for one year. After reconstitution, at 4˚C for one month. |
| It can also be aliquotted and stored frozen at -20˚C for a longer time. | |
| Avoid repeated freezing and thawing. | |
|
Tumor necrosis factor-alpha (TNFA) also kowns as TNFR1, It is a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. TNFR1 maps to 12p13. TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival. |
|
|
Concentration |
Tested Species |
Concluded Species |
Antigen Retrieval |
|
|
WB |
0.1-0.5μg/ml |
Hu, Ms |
- |
- |
|
IHC-P |
0.5-1μg/ml |
Hu, Rat, Ms |
- |
By Heat |
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