Isopropyl-beta-D-thiogalactoside (IPTG)

(isopropyl-β-D-thiogalactopyranoside; IPTG dioxane free)

Purity > 99.0 %

Molecular Biology Grade, synthetically made and not from animal origin

IPTG, dioxane free is an analog of lactose that inactivates the lac repressor resulting in IPTG induction. The lac repressor is a DNA binding protein that inhibits gene expression therefore you can think of IPTG operating by inhibiting an inhibitor thus allowing expression to occur. IPTG serves as a mimic of the naturally occurring allolactose that is an inducer of the lac operon.  This is commonly referred to as IPTG induction and used in protein expression. 

IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.

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IPTG Certification of Analysis

Stock Solution Preparation of 1 M IPTG (1 M IPTG Recipe)

1. Dissolve 2.38 g of IPTG in 8 mL of distilled H₂O.
2. Bring to a final volume of 10 mL with molecular biology grade H₂O.
3. Filter sterilize with a 0.22 μ syringe filter.
4. Store in 1mL aliquots at -20 °C.

View this method on protocols.io and use it directly at your bench

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